RESUMO
We report the first case of liver abscess due to Sterigmatomyces halophilus. Because this pathogen grows poorly in culture medium without added salts, it was identified by sequencing analysis targeting the rRNA gene internal transcribed spacer (ITS) region. This method could be useful for pathogens that cannot be cultured using standard methods.
Assuntos
Antifúngicos/uso terapêutico , Basidiomycota/isolamento & purificação , Abscesso Hepático/microbiologia , Micoses/microbiologia , Leucemia-Linfoma Linfoblástico de Células Precursoras/terapia , Anfotericina B/uso terapêutico , Antineoplásicos/efeitos adversos , Basidiomycota/imunologia , Biópsia , Transplante de Medula Óssea , Cefalosporinas/uso terapêutico , Neutropenia Febril Induzida por Quimioterapia/complicações , Neutropenia Febril Induzida por Quimioterapia/tratamento farmacológico , Neutropenia Febril Induzida por Quimioterapia/imunologia , Criança , Transplante de Células-Tronco Hematopoéticas , Humanos , Fígado/microbiologia , Fígado/patologia , Abscesso Hepático/diagnóstico , Abscesso Hepático/tratamento farmacológico , Abscesso Hepático/imunologia , Masculino , Micafungina/uso terapêutico , Micoses/diagnóstico , Micoses/tratamento farmacológico , Micoses/imunologia , Recidiva Local de Neoplasia/imunologia , Recidiva Local de Neoplasia/terapia , Leucemia-Linfoma Linfoblástico de Células Precursoras/imunologia , Resultado do Tratamento , CefozopranRESUMO
BACKGROUND: Nelarabine has been used for the treatment of T-cell malignancies including T-acute lymphoblastic leukemia (T-ALL)/T-lymphoblastic lymphoma. However, the mechanisms that underlie the susceptibility or resistance to nelarabine have not been fully elucidated. The aim of this study was to determine the significance of nelarabine transport and metabolism in the context of nelarabine cytotoxicity. PROCEDURE: The expression profiles of six genes in the nelarabine pathway were analyzed in blast cells from six patients with T-ALL as well as in three T-ALL cell lines. In vitro cytotoxicity (LC50 of 9-ß-d-arabinofuranosylguanine [ara-G]) was evaluated. RESULTS: The mRNA expression of ENT1, DCK, CDA, NT5C2, RRM1, and RRM2 in patients showed inter-individual variability and was not correlated with the LC50 of ara-G. However, the ratio of (ENT1 × DCK)/(CDA × RRM1) expression was significantly correlated with LC50 (r = -0.831, P = 0.0405). CONCLUSIONS: Chemosensitivity to nelarabine is influenced by the balance of the expression of these four genes, and the ratio of their expression predicts the response of T-cell malignancies to nelarabine.